Princess Beatrice and Princess Eugenie were absent from the Buckingham Palace balcony to watch the flypast this afternoon.
Prince Andrew's daughters were tipped to attend the monarch's official birthday as they have made more frequent appearances as of late.
However, the York sisters did not attend today's festivities, despite attending every year up until 2019.
Their father, the Duke of York, stepped down as a working royal five years ago after his career-ending Newsnight interview with Emily Maitlis.
Prince Andrew discussed his friendship with the late convicted sex offender, Jeffrey Epstein, which was met with huge backlash from the public.
As a result, the father-of-two was forced to step back from public life.
In 2022, Queen Elizabeth II made the decision to only allow working royals on the Buckingham Palace balcony during the Platinum Jubilee.
This would mean Andrew and his nephew Prince Harry would be barred from appearing beside the official working royals.
However, Beatrice, 35, and Eugenie, 34, were also implicated in this decision.
Before 2020, the York Princesses would always appear beside extended Royal Family members to show loyalty and support for the late monarch during Trooping the Colour.
Other non-working royals like Zara Tindall and Peter Phillips would also be present.
But King Charles III has not allowed non-working royals to return on the Buckingham Palace balcony as of yet.
LATEST ROYAL UPDATES:
Beatrice and Eugenie had been widely tipped by royal commentators to take on official working roles amid the Royal Family's current health crises.
Both the King and Princess Kate are currently battling cancer, meaning there are several gaps in the royal diary.
But King Charles has made it clear the York Princesses will not be enlisted as working royals.
The sisters have continued to support the Royal Family in a private capacity, having attended a Buckingham Palace garden party with Prince William last month.
